Ratiometric analysis of zidovudine (ZDV) incorporation by reverse transcriptases or polymerases via bio-orthogonal click chemistry.
نویسندگان
چکیده
A new fluorescence-based detection method was developed to visualize zidovudine-incorporated DNA using click chemistry. This bio-orthogonal detection method was used to quantify the relative susceptibility of various DNA-synthesizing enzymes toward ZDV incorporation on the basis of ratiometric analysis of band shifts in the gel and the visual observation of cellular DNA.
منابع مشابه
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عنوان ژورنال:
- Chemical communications
دوره 47 27 شماره
صفحات -
تاریخ انتشار 2011